BioMAJ3

This project is a complete rewrite of BioMAJ and the documentation is available here : http://biomaj.genouest.org.

BioMAJ (BIOlogie Mise A Jour) is a workflow engine dedicated to data synchronization and processing. The Software automates the update cycle and the supervision of the locally mirrored databank repository.

Common usages are to download remote databanks (Genbank for example) and apply some transformations (blast indexing, emboss indexing, etc.). Any script can be applied on downloaded data. When all treatments are successfully applied, bank is put in "production" on a dedicated release directory. With cron tasks, update tasks can be executed at regular interval, data are downloaded again only if a change is detected.

More documentation is available in wiki page.

BioMAJ is python 2 and 3 compatible.

Getting started

Edit global.properties file to match your settings. Minimal conf are database connection and directories.

biomaj-cli.py -h

biomaj-cli.py --config global.properties --status

biomaj-cli.py --config global.properties  --bank alu --update

Migration

To migrate from previous BioMAJ 1.x, a script is available at: https://github.com/genouest/biomaj-migrate. Script will import old database to the new database, and update configuration files to the modified format. Data directory is the same.

Migration for 3.0 to 3.1:

Biomaj 3.1 provides an optional micro service architecture, allowing to separate and distributute/scale biomaj components on one or many hosts. This implementation is optional but recommended for server installations. Monolithic installation can be kept for local computer installation. To upgrade an existing 3.0 installation, as biomaj code has been split into multiple components, it is necessary to install/update biomaj python package but also biomaj-cli and biomaj-daemon packages. Then database must be upgraded manually (see Upgrading in documentation).

To execute database migration:

python biomaj_migrate_database.py

Application Features

• Synchronisation:

• Multiple remote protocols (ftp, ftps, http, local copy, etc.)

• Data transfers integrity check

• Release versioning using a incremental approach

• Multi threading

• Data extraction (gzip, tar, bzip)

• Data tree directory normalisation

• Pre &Post processing :

• Advanced workflow description (D.A.G)

• Post-process indexation for various bioinformatics software (blast, srs, fastacmd, readseq, etc.)

• Easy integration of personal scripts for bank post-processing automation

• Supervision:

• Optional Administration web interface (biomaj-watcher)

• CLI management

• Mail alerts for the update cycle supervision

• Prometheus and Influxdb optional integration

• Optional consul supervision of processes

• Scalability:

• Monolithic (local install) or microservice architecture (remote access to a BioMAJ server)

• Microservice installation allows per process scalability and supervision (number of process in charge of download, execution, etc.)

• Remote access:

• Optional FTP server providing authenticated or anonymous data access

Dependencies

Packages:

• Debian: libcurl-dev, gcc
• CentOs: libcurl-devel, openldap-devel, gcc

Linux tools: tar, unzip, gunzip, bunzip

Database:

• mongodb (local or remote)

Indexing (optional):

• elasticsearch (global property, use_elastic=1)

ElasticSearch indexing adds advanced search features to biomaj to find bank having files with specific format or type. Configuration of ElasticSearch is not in the scope of BioMAJ documentation. For a basic installation, one instance of ElasticSearch is enough (low volume of data), in such a case, the ElasticSearch configuration file should be modified accordingly:

node.name: "biomaj" (or any other name)
index.number_of_shards: 1
index.number_of_replicas: 0

Installation

From source:

After dependencies installation, go in BioMAJ source directory:

python setup.py install

From packages:

pip install biomaj biomaj-cli biomaj-daemon

You should consider using a Python virtual environment (virtualenv) to install BioMAJ.

In tools/examples, copy the global.properties and update it to match your local installation.

The tools/process contains example process files (python and shell).

Docker

You can use BioMAJ with Docker (genouest/biomaj)

docker pull genouest/biomaj
docker pull mongo
docker run --name biomaj-mongodb -d mongo
# Wait ~10 seconds for mongo to initialize
# Create a local directory where databases will be permanently stored
# *local_path*
docker run --rm -v local_path:/var/lib/biomaj --link biomaj-mongodb:biomaj-mongodb osallou/biomaj-docker --help

Copy your bank properties in directory local_path/conf and post-processes (if any) in local_path/process

You can override global.properties in /etc/biomaj/global.properties (-v xx/global.properties:/etc/biomaj/global.properties)

No default bank property file or process are available in the container.

Examples are available at https://github.com/genouest/biomaj-data

Import bank templates

Once biomaj is installed, it is possible to import some bank examples with the biomaj client

# List available templates
biomaj-cli ... --data-list
# Import a bank template
biomaj-cli ... --data-import --bank alu
# then edit bank template in config directory if needed and launch bank update
biomaj-cli ... --update --bank alu

API documentation

https://readthedocs.org/projects/biomaj/

Status

Testing

Execute unit tests

nosetests

Execute unit tests but disable ones needing network access

nosetests -a '!network'

Monitoring

InfluxDB (optional) can be used to monitor biomaj. Following series are available:

• biomaj.banks.quantity (number of banks)
• biomaj.production.size.total (size of all production directories)
• biomaj.workflow.duration (workflow duration)
• biomaj.production.size.latest (size of latest update)
• biomaj.bank.update.downloaded_files (number of downloaded files)
• biomaj.bank.update.new (track updates)

WARNING Influxdb database must be created, biomaj does not create the database (see https://docs.influxdata.com/influxdb/v1.6/query_language/database_management/#create-database)

License

A-GPL v3+

Remarks

To delete elasticsearch index:

curl -XDELETE 'http://localhost:9200/biomaj_test/'

Credits

Special thanks for tuco at Pasteur Institute for the intensive testing and new ideas. Thanks to the old BioMAJ team for the work they have done.

BioMAJ is developped at IRISA research institute.

3.1.12: In case of multiple matches for release regexp, try to determine most recent one #115 Correctly use save_as for release file name 3.1.11: Increase one log level #110 Allow ftps and directftps protocols (needs biomaj-download 3.0.26 and biomaj-core 3.0.19) #111 locked bank after bad update command Ignore UTF-8 errors in release file Add plugin support via biomaj-plugins repo (https://github.com/genouest/biomaj-plugins) to get release and list of files to download from a plugin script. Add support for protocol options in global and bank properties (options.names=x,y options.x=val options.y=val). Options may be ignored or used differently depending on used protocol. 3.1.10: Allow to use hardlinks when reusing files from previous releases 3.1.9: Fix remote.files recursion 3.1.8: Fix uncompress when saved files contains subdirectory 3.1.7: Fix utf/ascii encoding issue with python3 In case of uncompress failure, put back all compressed files to avoid redownload 3.1.6: Fix #100 Catch error and log error if biomaj fails to connect to InfluxDB Add history to update/remove operations Add log in case of file deletion error during bank removal check lock file exists when removing it Update protobuf to work with biomaj.download 3.0.18

3.1.5: Fix #97 Wrong offline dir checks

3.1.4: Fix #88 Unset 'last_update_session' when found in pending sessions using --remove-pending Add formats in bank info request Add checks for some production fields before display Add irods download support

3.1.3: Remove post-install step for automatic upgrades, not supported by wheel package

3.1.2: Fix #86 remove special character from README.md Feature #85 SchemaVersion automatically add new property

3.1.1: Fix #80 Check process exists with --from-task and --process Manage old banks with no status

3.1.0:

Needs database upgrade

If using biomaj-watcher, must use version >= 3.1.0 Feature #67,#66,#61 switch to micro service architecture. Still works in local monolithic install Fix some configuration parameter loading when not defined in config Fix HTTP parsing parameters loading Fix download_or_copy to copy files in last production release if available instead of downloading files again Manage user migration for micro services Feature #74 add influxdb statistics Feature #65 add a release info file at the root of the bank which can be used by other services to know the latest release available Feature #25 experimental support of rsync protocol Add rate limiting for download with micro services Limit email size to 2Mb, log file may be truncated

3.0.20: Fix #55: Added support for https and directhttps Add possibility to define files to download from a local file with remote.list parameter Fix visibility modification (bug deleted the bank properties field) Fix #65 Add release file in bank dir after update Add md5 or sha256 checksum checks if files are downloaded and available

3.0.19: Fix missing README.md in package Fix #53 avoid duplicates in pending databases

3.0.18: Add migration method to update schema when needed Manage HTTP month format to support text format (Jan, Feb, ...) and int format (01, 02, ...) New optional bank property http.parse.file.date.format to extract date in HTTP protocol following python date regexp format (http://www.tutorialspoint.com/python/time_strptime.htm) Example: %d-%b-%Y %H:%M

3.0.17: Fix #47: save_as error with directhttp protocol Fix #45: error with pending releases when release has dots in value typo/pylint fixes

3.0.16: Do not use config values, trust database values #39 Fix #42: Add optional release.separator to name the bank directory bankname_release (underscore as default)

3.0.15: Fix #37: remote local files history from db and put it in cache.dir Feature #38: add optional keep.old.sessions parameter to keep all sessions in database, even for removed releases Feature #28: add optional release.format parameter to specify the date format of a release

3.0.14: Fix in method set_owner Force release to be a str Fix #32: fix --from-task issue when calling a meta process Fix #34: remove release from pending when doing cleanup of old sessions Remove logs on some operations Add --status-ko option to list bank in error state Fix #36 manage workflows over by error or unfinished

3.0.13: Fix #27: Thread lock issue during download New optional attribute in bank properties: timeout.download HTTP protocol fix (deepcopy error)

3.0.12: Fix index deletion on bank removal Fix lock errors on dir creation for multi-threads, pre-create directroy structure in offline directory Fix #26: save error when too many files in bank

3.0.11: Fix in session management with pre and rm processes Fix #23: Check workflow step name passed to --stop-after/--start-after/--from-task Fix #24: deprecated delete_by_query method in elasticsearch Add some controls on base directories

3.0.10: Change dir to process.dir to find processes in subdirs If all files found in offline dir, continue workflow with no download Remove extra log files for bank dependencies (computed banks) Fix computed bank update when sub banks are not updated Fix #15 when remote reverts to a previous release Feature #16: get possibility not to download files (for computed banks for example). Set protocol='none' in bank properties. Fix on --check with some protocols Fix #21 release.file not supported for directhttp protocol Feature #22: add localrelease and remoterelease bank properties to use the remote release as an expression in other properties => remote.dir = xx/yy/%(remoterelease)s/zz Feature #17,#20: detect remote modifications even if release is the same new parameter release.control (true, false) to force a check even if remote release (file controlled or date) is the same. Fix on 'multi' protocol Fix on "save_as" regexp when remote.files starts with a ^ character.

3.0.9: Fix thread synchro issue: during download some download threads could be alive while main thread continues worflow the fix prevents using Ctrl-C during download Workflow fix: if subtask of workflow fails, fail main task

3.0.8: do not test index if elasticsearch is not up minor fixes add http proxy support pylint fixes retry uncompress once in case of failure (#13)

3.0.7: Reindent code, pep8 fixes Various fixes on var names and OrderedDict suport for Python < 2.7 Merge config files to be able to reference global.properties variables in bank property file in format %(xx)s Use ConfigParser instead of SafeConfigParser that will be deprecated

3.0.6: Add option --remove-pending to remove all pending sessions and directories Add process env variables logdir and logfile Fix Unicode issue with old versions of PyCurl.

3.0.5: Fix removal workflow during an update workflow, removedrelease was current release. Fix shebang of biomaj-cli, and python 2/3 compat issue

3.0.4: Update code to make it Python 3 compatible Use ldap3 library (pure Python and p2,3 compatible) instead of python-ldap get possiblity to save downloaded files for ftp and http without keeping full directory structure: remote.files can include groups to save file without directory structure, or partial directories only, examples: remote.files = genomes/fasta/..gz => save files in offline directory, keeping remote structure offlinedir/genomes/fasta/ remote.files = genomes/fasta/(..gz) => save files in offline directory offlinedir/ remote.files = genomes/(fasta)/(.*.gz) => save files in offline directory offlinedir/fasta