leicaexperiment 0.1.1

Overview

This is a python module for interfacing with Leica LAS AF/X Matrix Screener experiments.

The module can be used to:

• stitch wells from an experiment exported with the LAS AF Data Exporter

• batch compress images lossless

• programmatically select slides/wells/fields/images given by attributes like

  • slide (S)

  • well position (U, V)

  • field position (X, Y)

  • z-stack position (Z)

  • channel (C)

Features

• Access experiment as a python object

• Compress to PNGs without loosing precision, metadata or colormap

• ImageJ stitching (Fiji is installed via fijibin)

Installation

Install using pip…

pip install leicaexperiment

Examples

from leicaexperiment import Experiment

# path should contain AditionalData and slide--S*
experiment = Experiment('path/to/experiment')

# if path is omitted, experiment path is used for output files
stitched_images = experiment.stitch('/path/to/output/files/')

# get information about placement of images in the stitch
xs, ys, attrs = experiment.stitch_coordinates(well_x=0, well_y=0)

from leicaexperiment import Experiment

# path should contain AditionalData and slide--S*
stitched_images = experiment.stitch('/path/to/well')

from leicaexperiment import Experiment

experiment = Experiment('path/to/experiment--')

for image in experiment.images:
    do stuff...

from leicaexperiment import attribute

# select specific parts
selected_wells = [well for well in experiment.wells if 'U00' in well]
for well in selected_wells:
    do stuff...

def condition(path):
    x_above = attribute(path, 'X') > 1
    x_below = attribute(path, 'X') < 5
    return x_above and x_below

selected_fields = [field for field in experiment.fields if condition(field)]
for field in selected_fields:
    do stuff..

from leicaexperiment import attribute

# get all channels
channels = [attribute(image, 'C') for image in experiment.images]
min_ch, max_ch = min(channels), max(channels)

from leicaexperiment import Experiment, compress

e = Experiment('/path/to/experiment')
pngs = compress(e.images)
print(pngs)

API reference

API reference is at http://leicaexperiment.rtfd.org.

Development

Install dependencies and link development version of leicaexperiment to pip:

git clone https://github.com/arve0/leicaexperiment
cd leicaexperiment
pip install -r requirements.txt

pip install tox
tox

DEBUG=leicaexperiment tox -- --pdb -s

make docs