Add an air bubbler to your Pioreactor as a background job
Project description
Pioreactor air bubbler
Add an air-pump / bubbler to your Pioreactor. This pump can run continuously, or if OD reading is running, will stop during a reading.
Usage
pio run air_bubbler
Installation
Software
From the command line, run:
pio install-plugin pioreactor_air_bubbler
(Optional) Edit the following to your config.ini
[PWM]
<the PWM channel you pick>=air_bubbler
[air_bubbler.config]
duty_cycle=10
hertz=200
pre_delay_duration=1.5
post_delay_duration=0.75
enable_dodging_od=1
Hardware
- Connect the PWM channel to the air pump's power source.
- Connect a tube between the air pump and a tube in the vial's cap, via luer lock.
- The connecting tube in the vial cap can be pushed into the liquid for bubbling, or left in the headspace to exchange air.
- Optional: a 0.22 micron filter can be placed along the air path to filter contaminants.
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