Normalization of RNA-seq gene expression.
Project description
Normalization of RNA-seq gene expression. Supported methods: Transcript per kilobase million (TPM) and Counts per million (CPM).
Usage
Install rnanorm Python package:
pip install rnanorm
See rnanorm command help:
rnanorm --help
Run rnanorm with pre-computed gene lengths:
rnanorm expr.tsv --cpm-output=expr.cpm.tsv --tpm-output=expr.tpm.tsv --gene-lengths=lengths.tsv
Run rnanorm with genome annotation - gene lengths will be computed on the fly:
rnanorm expr.tsv --cpm-output=expr.cpm.tsv --tpm-output=expr.tpm.tsv --annotation=annot.gtf
Contributing
Install rnanorm Python package for development:
flit install --deps=all --symlink
Run all tests and linters:
tox
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